2 UpdatePanels and 1 UpdatePanelAnimationExtender

Try to take a look at this reading about UpdatePanelAnimationExtender -?http://ajax.asp.net/ajaxtoolkit/UpdatePanelAnimation/UpdatePanelAnimation.aspx
Besides,can you paste some of your codes about how do you call javascript after the UpdatePanel1 was updated here?

I know about this article and I know that UpdatePanelAnimationExtender will fire every time that one of all UpdatePanels was updated. And I want solve this trouble

this code call javascript after the UpdatePanel1 was updated

<ajaxToolkit:UpdatePanelAnimationExtender ID="ae" runat="server" TargetControlID="UpdatePanel1">
<Animations>
<OnUpdated>
<Sequence>
<ScriptAction Script="RunAction();" />
</Sequence>
</OnUpdated>
</Animations>
</ajaxToolkit:UpdatePanelAnimationExtender>

You can have a look at this reading about Ajax control toolkit - http://weblogs.asp.net/scottgu/archive/2006/09/19/September-Atlas-Control-Toolkit-Released-_2800_Now-with-Animation-Support_2900_.aspx
Wish this can give you some helps.